6IWV
Crystal structure of a single strand DNA binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2017-12-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 116.906, 116.906, 42.355 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.520 |
| R-factor | 0.14733 |
| Rwork | 0.146 |
| R-free | 0.18055 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.247 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.550 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rpim | 0.015 | 0.728 |
| Number of reflections | 26784 | 1288 |
| <I/σ(I)> | 94.73 | |
| Completeness [%] | 99.1 | |
| Redundancy | 22.8 | |
| CC(1/2) | 1.000 | 0.626 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 293 | 0.1 M Bis-Tris pH 6.3, 1.9 M Ammonium sulfate |
