6IWO

Structural insight into probable lipid transfer mechanism of non-specific lipid transfer protein via intermediate structures in Solanum melongena

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-10-04
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	P 61
Unit cell lengths	87.691, 87.691, 50.689
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.160 - 2.350
R-factor	0.206
Rwork	0.204
R-free	0.24450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6iwm
RMSD bond length	0.004
RMSD bond angle	0.736
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((dev_2747: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.430
High resolution limit [Å]	2.350	2.350
Rmerge	0.118	0.590
Number of reflections	9383	931
<I/σ(I)>	18.01	2.88
Completeness [%]	99.8	99.5
Redundancy	8.2	6.1
CC(1/2)	0.997	0.591

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295.15	0.1M HEPES pH 8.0, 2M Ammonium sulfate