6IWN
Structural insight into probable lipid transfer mechanism of non-specific lipid transfer protein via intermediate structures in Solanum melongena
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-09-23 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97625 |
Spacegroup name | P 61 |
Unit cell lengths | 86.349, 86.349, 50.440 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.390 - 2.408 |
R-factor | 0.2268 |
Rwork | 0.225 |
R-free | 0.25360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6iwm |
RMSD bond length | 0.004 |
RMSD bond angle | 0.714 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2747: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.116 | 0.412 |
Number of reflections | 8149 | 706 |
<I/σ(I)> | 14 | 2.26 |
Completeness [%] | 96.9 | 88.2 |
Redundancy | 3.3 | 2.7 |
CC(1/2) | 0.892 | 0.728 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 295.15 | 0.1M HEPES pH 7.2, 2M Ammonium sulfate |