6IW5

Crystal structure of YFV-China sE in prefusion state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 1 21 1
Unit cell lengths	65.785, 121.362, 101.581
Unit cell angles	90.00, 93.46, 90.00

Refinement procedure

Resolution	46.780 - 2.500
R-factor	0.2393
Rwork	0.236
R-free	0.29160
RMSD bond length	0.006
RMSD bond angle	1.313
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	50.000
High resolution limit [Å]	2.500	2.500
Number of reflections	55335
<I/σ(I)>	13.8
Completeness [%]	99.4
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	0.1 M Tris pH 8.5, 25% w/v Polyethylene glycol 3350