6IW4

Crystal structure of YFV-17D sE in prefusion state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 63
Unit cell lengths	150.361, 150.361, 88.898
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.217 - 2.801
R-factor	0.2444
Rwork	0.243
R-free	0.27640
RMSD bond length	0.005
RMSD bond angle	0.898
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	49.200
High resolution limit [Å]	2.800	2.800
Number of reflections	23443
<I/σ(I)>	0.7
Completeness [%]	100.0
Redundancy	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	0.1 M HEPES pH 7.0, 12% w/v Polyethylene glycol 3350