6IVP
Crystal structure of a membrane protein P262A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-04-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 114.482, 162.411, 161.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.010 - 3.800 |
| R-factor | 0.26809 |
| Rwork | 0.266 |
| R-free | 0.31546 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.161 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 162.400 | 4.030 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.090 | 0.887 |
| Rpim | 0.057 | 0.570 |
| Number of reflections | 29684 | 4728 |
| <I/σ(I)> | 7.7 | 1.8 |
| Completeness [%] | 98.2 | 99.1 |
| Redundancy | 3.3 | 3.3 |
| CC(1/2) | 0.506 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.05 M zinc acetate, 6% v/v ethylene glycol, 0.1 M sodium cacodylate, pH 5.0, 7.5 % w/v PEG 8000 |
