6IVO

Crystal structure of a membrane protein P208A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2017-12-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	114.009, 160.062, 161.843
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.760 - 2.450
R-factor	0.20406
Rwork	0.202
R-free	0.23816
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.288
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	160.100	2.490
High resolution limit [Å]	2.450	2.450
Rmerge	0.067	0.977
Rpim	0.028	0.399
Number of reflections	109263	5365
<I/σ(I)>	17.7	2.2
Completeness [%]	100.0	100
Redundancy	6.8	6.9
CC(1/2)		0.755

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	0.01 M zinc acetate, 6% v/v ethylene glycol, 0.1 M sodium cacodylate, pH 6.0, 6.6 % w/v PEG 8000