6IVN
Crystal structure of a membrane protein G264A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-02-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 114.149, 160.939, 160.957 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.560 - 3.100 |
| R-factor | 0.21562 |
| Rwork | 0.213 |
| R-free | 0.26482 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.753 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 160.900 | 3.200 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.058 | 0.730 |
| Rpim | 0.036 | 0.457 |
| Number of reflections | 53373 | 4601 |
| <I/σ(I)> | 13.2 | |
| Completeness [%] | 98.4 | 99.5 |
| Redundancy | 3.4 | 3.4 |
| CC(1/2) | 0.610 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.05 M zinc acetate, 6% v/v ethylene glycol, 0.1 M sodium cacodylate, pH 6.0, 6.6 % w/v PEG 8000 |
