6IVM
Crystal structure of a membrane protein P143A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 113.965, 158.808, 161.682 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.720 - 2.950 |
| R-factor | 0.20946 |
| Rwork | 0.208 |
| R-free | 0.24291 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.406 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 158.820 | 3.030 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.087 | 0.907 |
| Rpim | 0.083 | 0.540 |
| Number of reflections | 60739 | 4465 |
| <I/σ(I)> | 11.1 | |
| Completeness [%] | 97.7 | 98.8 |
| Redundancy | 3.4 | 3.5 |
| CC(1/2) | 0.666 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.05 M zinc acetate, 6% v/v ethylene glycol, 0.1 M sodium cacodylate, pH 6.0, 6.6 % w/v PEG 8000 |
