6IUT
Crystal structure of influenza A virus H5 hemagglutinin globular head in complex with the Fab of antibody AVFluIgG01
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-09-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9796 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 112.101, 150.046, 107.968 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.066 - 2.300 |
R-factor | 0.2075 |
Rwork | 0.205 |
R-free | 0.25520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5dut |
RMSD bond length | 0.009 |
RMSD bond angle | 1.101 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.350 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.140 | 0.957 |
Number of reflections | 39949 | 3508 |
<I/σ(I)> | 13.8 | |
Completeness [%] | 99.0 | |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 25% w/v Polyethylene glycol 1500 |