6IUS
A higher kcat Rubisco
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 166.501, 107.842, 112.306 |
| Unit cell angles | 90.00, 130.04, 90.00 |
Refinement procedure
| Resolution | 46.990 - 2.120 |
| R-factor | 0.19319 |
| Rwork | 0.192 |
| R-free | 0.23074 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5c2c |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.647 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.160 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.138 | 0.396 |
| Rpim | 0.056 | 0.166 |
| Number of reflections | 86160 | |
| <I/σ(I)> | 25.4 | 3.4 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 6.8 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.15M Magnesium formate, 12%(w/v) Polyethylene glycol 3,350 |
