6IUQ
Crystal structure and expression patterns of prolyl 4-hydroxylases from Phytophthora capsici
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.724, 63.782, 72.845 |
| Unit cell angles | 80.18, 65.27, 71.17 |
Refinement procedure
| Resolution | 43.669 - 2.348 |
| R-factor | 0.1861 |
| Rwork | 0.184 |
| R-free | 0.21680 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.098 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.390 |
| High resolution limit [Å] | 2.348 | 2.350 |
| Rpim | 0.029 | 0.172 |
| Number of reflections | 38776 | 1882 |
| <I/σ(I)> | 8.7 | |
| Completeness [%] | 98.0 | |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 283 | 0.1M sodium citrate tribasic dehydrate pH5.0, 18% (w/v) PEG20000 |
