6IUC

Structure of Helicobacter pylori Soj-ATP complex bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	293
Detector technology	CCD
Collection date	2017-04-20
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	74.418, 74.628, 80.351
Unit cell angles	71.38, 71.56, 67.83

Refinement procedure

Resolution	26.786 - 3.400
R-factor	0.205
Rwork	0.202
R-free	0.26340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6iub
RMSD bond length	0.016
RMSD bond angle	1.641
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.520
High resolution limit [Å]	3.400	3.400
Rmerge	0.133	0.419
Number of reflections	18173	2049
<I/σ(I)>	8.5
Completeness [%]	88.0	98.6
Redundancy	2	2
CC(1/2)		0.717

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	MES (pH 5.6), KCl, MgCl2, PEG 8000