6ITU

Crystal Structure of the GULP1 PTB domain-APP peptide complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2011-09-08
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5419
Spacegroup name	P 32 2 1
Unit cell lengths	63.910, 63.910, 108.771
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.000 - 2.170
R-factor	0.19779
Rwork	0.197
R-free	0.22081
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ntv
RMSD bond length	0.014
RMSD bond angle	1.678
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0171)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.000	2.290
High resolution limit [Å]	2.170	2.170
Rmerge	0.056	0.369
Number of reflections	13797	1945
<I/σ(I)>	26.8
Completeness [%]	98.4
Redundancy	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.3	289.15	100 mM Glycine (pH3.3), 2000 mM (NH4)2SO4, 50 mM NaCl and 50 mM KCl