6ITA

Crystal structure of intracellular B30.2 domain of BTN3A1 mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-11-09
Detector	RAYONIX MX-300
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	46.961, 46.961, 158.996
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.040 - 1.200
R-factor	0.20425
Rwork	0.203
R-free	0.23186
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4n7u
RMSD bond length	0.016
RMSD bond angle	1.721
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.040	1.240
High resolution limit [Å]	1.200	1.200
Rmerge	0.039	0.452
Number of reflections	52887	5020
<I/σ(I)>	57.27
Completeness [%]	98.6
Redundancy	8.9
CC(1/2)		0.964

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	Sodium Fluoride, Polyethylene Glycol 3350