6IST
Crystal structure of a wild type endolysin LysIME-EF1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-04-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97981 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.030, 56.920, 91.520 |
| Unit cell angles | 90.00, 96.29, 90.00 |
Refinement procedure
| Resolution | 90.970 - 1.750 |
| R-factor | 0.18198 |
| Rwork | 0.180 |
| R-free | 0.22349 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.779 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.970 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.160 | |
| Rmeas | 0.173 | |
| Number of reflections | 47634 | 3471 |
| <I/σ(I)> | 2 | |
| Completeness [%] | 98.0 | 98.5 |
| Redundancy | 6.7 | |
| CC(1/2) | 0.992 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 20% PEG 3350 0.1M Tris ,pH 8.5 0.2 M NaCl |
