6IS8

Crystal structure of ZmMoc1 D115N mutant in complex with Holliday junction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.978
Spacegroup name	P 1 21 1
Unit cell lengths	53.699, 77.907, 63.577
Unit cell angles	90.00, 97.55, 90.00

Refinement procedure

Resolution	49.000 - 1.680
R-factor	0.1595
Rwork	0.157
R-free	0.20390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6is9
RMSD bond length	0.007
RMSD bond angle	0.796
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.740
High resolution limit [Å]	1.680	3.620	1.680
Rmerge	0.057	0.045	0.765
Rmeas	0.062	0.048	0.829
Rpim	0.024	0.019	0.317
Number of reflections	58742	5976	5827
<I/σ(I)>	8.4
Completeness [%]	99.5	99.2	99.5
Redundancy	6.7	6.6	6.7
CC(1/2)		0.997	0.811

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	298	0.1 M Bis-tris pH 7.5, 37.5% PEG 400