6IS3
Crystal Structure of Staphylococcus aureus response regulator ArlR receiver domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.375, 41.208, 42.877 |
| Unit cell angles | 97.50, 118.85, 96.06 |
Refinement procedure
| Resolution | 33.000 - 1.549 |
| R-factor | 0.1756 |
| Rwork | 0.173 |
| R-free | 0.20560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.024 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.000 | 1.600 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 28417 | |
| <I/σ(I)> | 21.36 | |
| Completeness [%] | 87.3 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Calcium chloride dehydrate, 0.1M MES and 20% w/v Polyethylene glycol 3,350 |
