6IRI
Crystal structure of the minor ferredoxin from Thermosynechococcus elongatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-01-31 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 27.946, 51.121, 31.570 |
| Unit cell angles | 90.00, 116.15, 90.00 |
Refinement procedure
| Resolution | 28.340 - 1.380 |
| R-factor | 0.1146 |
| Rwork | 0.112 |
| R-free | 0.15700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5aui |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.086 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 28.340 | 28.340 | 1.470 |
| High resolution limit [Å] | 1.380 | 4.110 | 1.380 |
| Rmerge | 0.056 | 0.585 | |
| Number of reflections | 16325 | 2593 | |
| <I/σ(I)> | 12.82 | 2 | |
| Completeness [%] | 99.3 | 98.9 | |
| Redundancy | 3.68 | 3.62 | |
| CC(1/2) | 0.999 | 0.718 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 100 mM HEPES (pH 7.5), 2.4 M ammonium sulfate |
