6IR6
Green fluorescent protein variant GFPuv with the native lysine residue at the C-terminus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.586, 50.951, 47.322 |
| Unit cell angles | 90.00, 98.49, 90.00 |
Refinement procedure
| Resolution | 35.946 - 1.642 |
| R-factor | 0.189 |
| Rwork | 0.188 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b9c |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.075 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.680 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Number of reflections | 26115 | 1282 |
| <I/σ(I)> | 27.4 | 1.8 |
| Completeness [%] | 95.7 | 96.5 |
| Redundancy | 3.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30%(w/v) PEG 1500, 3%(v/v) MPD, 0.2M Magnesium sulfate, 0.1M Sodium acetate/Acetic acid pH 5.5 |
