6IQX
High resolution structure of bilirubin oxidase from Myrothecium verrucaria - M467Q mutant, aerobically prepared
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-16 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.242, 152.579, 69.995 |
| Unit cell angles | 90.00, 91.69, 90.00 |
Refinement procedure
| Resolution | 34.063 - 1.432 |
| R-factor | 0.1615 |
| Rwork | 0.161 |
| R-free | 0.19940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xll |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.180 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3235: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.450 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmerge | 0.119 | |
| Rpim | 0.076 | |
| Number of reflections | 225552 | |
| <I/σ(I)> | 12.13 | |
| Completeness [%] | 99.5 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M MES pH 5.5, 12% (w/v) PEG 8000, 0.1M calcium acetate |
