6IQT

Crystal Structure of CagV, a VirB8 homolog of T4SS from Helicobacter pylori Strain 26695

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AichiSR BEAMLINE BL2S1
Synchrotron site	AichiSR
Beamline	BL2S1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-18
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	67.026, 96.851, 99.148
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.574 - 1.922
R-factor	0.1854
Rwork	0.184
R-free	0.21080
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	0.860
Data reduction software	HKL-2000
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.960
High resolution limit [Å]	1.922	5.240	1.930
Rmerge	0.161	0.092	2.092
Rmeas	0.167	0.096	2.274
Rpim	0.046	0.028	0.850
Number of reflections	49731	2728	1629
<I/σ(I)>	10.5
Completeness [%]	98.2	99.3	65.1
Redundancy	13.2	12.2	5.7
CC(1/2)		0.994	0.301

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	289	10% PEG2000MME(v/v), 0.3M trimethymaline n-oxidedehydrate, 0.1M Tris-HCl pH 8.5 buffer