6IQR
Crystal structure of Prc with S452I and L252Y mutations
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2018-06-07 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 129.277, 129.277, 236.566 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.970 - 3.420 |
| R-factor | 0.24193 |
| Rwork | 0.239 |
| R-free | 0.28758 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5wql |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.788 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 (v712) |
| Phasing software | PHASER (2.70) |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.540 |
| High resolution limit [Å] | 3.420 | 3.420 |
| Rmerge | 0.075 | 0.651 |
| Rmeas | 0.145 | 0.942 |
| Rpim | 0.069 | 0.446 |
| Number of reflections | 31494 | 3117 |
| <I/σ(I)> | 13.8 | 2.1 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 4.3 | 4.5 |
| CC(1/2) | 0.772 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | PEG3350, sodium thyocinate |
