6IQM
Crystal Structure of Cell Surface Glyceraldehyde-3-Phosphate Dehydrogenase Complexed with NAD+ from Lactobacillus plantarum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-26 |
Detector | DECTRIS PILATUS3 S 2M |
Wavelength(s) | 1.0000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 126.207, 171.953, 149.752 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 101.740 - 1.850 |
R-factor | 0.16012 |
Rwork | 0.159 |
R-free | 0.19045 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5j9g |
RMSD bond length | 0.022 |
RMSD bond angle | 2.155 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 101.740 | 1.880 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.045 | 0.315 |
Number of reflections | 137453 | |
<I/σ(I)> | 27.3 | |
Completeness [%] | 99.1 | 96.9 |
Redundancy | 9.4 | 8.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 25% (w/v) PEG 1500, 0.1M MIB buffer (pH 5.0) |