6IQJ

crystal structure of Arabidopsis thaliana Profilin 2 complex with formin1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-X
Temperature [K]	77
Detector technology	PIXEL
Collection date	2017-05-12
Detector	DECTRIS PILATUS 300K
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	51.523, 65.156, 73.533
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.414 - 1.922
R-factor	0.1851
Rwork	0.183
R-free	0.21780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3nul
RMSD bond length	0.003
RMSD bond angle	0.581
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.414	1.991
High resolution limit [Å]	1.920	1.923
Number of reflections	18928	759
<I/σ(I)>	9.3
Completeness [%]	97.4
Redundancy	10
CC(1/2)	0.998	0.793

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		291.15	0.1M Bis-tris (pH 7.0), 2.0M ammonium phosphate dibasic