6IQI

crystal structure of Arabidopsis thaliana Profilin 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-X
Temperature [K]	77
Detector technology	PIXEL
Collection date	2017-03-28
Detector	DECTRIS PILATUS 300K
Wavelength(s)	1.54
Spacegroup name	P 32
Unit cell lengths	58.680, 58.680, 83.938
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.700 - 2.400
R-factor	0.2656
Rwork	0.262
R-free	0.29670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3nul
RMSD bond length	0.006
RMSD bond angle	0.920
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.360	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	19448	719
<I/σ(I)>	6
Completeness [%]	98.9
Redundancy	4.2
CC(1/2)	0.996	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		291.15	0.1M Tris HCl (pH 8.5), 2.2M ammonium phosphate dibasic