6IQG
X-ray crystal structure of Fc and peptide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.062, 60.507, 69.526 |
| Unit cell angles | 90.00, 101.25, 90.00 |
Refinement procedure
| Resolution | 35.034 - 2.998 |
| R-factor | 0.2017 |
| Rwork | 0.198 |
| R-free | 0.27290 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1dn2 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.588 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.220 | 3.110 |
| High resolution limit [Å] | 2.998 | 3.000 |
| Rmerge | 0.138 | 0.564 |
| Number of reflections | 10959 | 1071 |
| <I/σ(I)> | 9.5 | 2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 293 | PEG 3350, KI |
