6IQC
Wild-type Programmed Cell Death 5 protein from Sulfolobus solfataricus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-03-17 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 75.094, 36.634, 36.197 |
| Unit cell angles | 90.00, 98.40, 90.00 |
Refinement procedure
| Resolution | 23.480 - 1.490 |
| R-factor | 0.21647 |
| Rwork | 0.210 |
| R-free | 0.27791 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6iqo |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.812 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.480 | 1.550 |
| High resolution limit [Å] | 1.490 | 1.500 |
| Rmerge | 0.062 | 0.563 |
| Rpim | 0.015 | 0.316 |
| Number of reflections | 15594 | 1552 |
| <I/σ(I)> | 48.25 | |
| Completeness [%] | 98.1 | |
| Redundancy | 4.1 | |
| CC(1/2) | 0.891 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 6.9 | 293 | 20% PEG 3350, 0.2M KSCN |
