6IQ6
Crystal structure of GAPDH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-07-26 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.630, 108.490, 175.810 |
| Unit cell angles | 90.00, 96.37, 90.00 |
Refinement procedure
| Resolution | 35.870 - 2.290 |
| R-factor | 0.21732 |
| Rwork | 0.215 |
| R-free | 0.25505 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wnc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.397 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.520 | 2.330 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.101 | 0.358 |
| Number of reflections | 114488 | 24713 |
| <I/σ(I)> | 9 | |
| Completeness [%] | 99.7 | |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.1 M Sodium Acetate, 0.1 M HEPES-NaOH pH 7.5, 22 % (w/v) PEG 4000, 4.4 % (v/v) Formamide |
