6IPX

Crystal structure of the apo form transcription factor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-01-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97981
Spacegroup name	P 21 21 2
Unit cell lengths	120.460, 214.710, 33.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	107.355 - 2.634
R-factor	0.2334
Rwork	0.230
R-free	0.28210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6IM2
RMSD bond length	0.010
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	107.355	2.700
High resolution limit [Å]	2.634	2.630
Rmerge	0.075	0.075
Number of reflections	49620	1866
<I/σ(I)>	2
Completeness [%]	99.2
Redundancy	6.5
CC(1/2)		0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289.15	20% PEG 4K, 50 mM magnesium acetate