6IPP

Non-native ferritin 8-mer mutant-C90A/C102A/C130A/D144C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-05-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9779
Spacegroup name	F 4 3 2
Unit cell lengths	235.374, 235.374, 235.374
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.046 - 2.699
R-factor	0.1962
Rwork	0.194
R-free	0.23210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fha
RMSD bond length	0.010
RMSD bond angle	0.997
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.050	3.105
High resolution limit [Å]	2.699	2.699
Rmerge	0.194
Number of reflections	15943
<I/σ(I)>	6.5
Completeness [%]	100.0
Redundancy	11.3
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	293.17	Sodium chloride,MPD,TRIS