6IPO

Ferritin mutant C90A/C102A/C130A/D144C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-05-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9779
Spacegroup name	F 4 3 2
Unit cell lengths	236.229, 236.229, 236.229
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.050 - 2.998
R-factor	0.1938
Rwork	0.191
R-free	0.24530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fha
RMSD bond length	0.009
RMSD bond angle	0.951
Data reduction software	HKL-2000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.050	2.998
High resolution limit [Å]	2.699	2.796
Rmerge	0.191
Number of reflections	11867
<I/σ(I)>	8.1
Completeness [%]	100.0
Redundancy	12.3
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293.15	magnesium chloride, PEG 400, TRIS