6IPK

Binary Complex of Human DNA Polymerase Mu with Mn8oxodGTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-19
Detector	RAYONIX MX-225
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	122.827, 122.827, 49.859
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.150
R-factor	0.21509
Rwork	0.213
R-free	0.25623
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.437
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.280
High resolution limit [Å]	2.150	2.150
Rmeas	0.060	0.364
Number of reflections	21351	3375
<I/σ(I)>	25.9	5.78
Completeness [%]	99.9	99.8
Redundancy	13.32	13.88
CC(1/2)	0.999	0.979

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1 M HEPES pH 7.5, 1.5 M Li2SO4