6IPH

Binary Complex of Human DNA Polymerase Mu with MndATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-02
Detector	RAYONIX MX300-HS
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	124.582, 124.582, 50.548
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.650
R-factor	0.189
Rwork	0.188
R-free	0.21560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.358
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.750
High resolution limit [Å]	1.650	1.650
Rmeas	0.087	0.382
Number of reflections	48180	7615
<I/σ(I)>	14.99	4.25
Completeness [%]	99.5	99
Redundancy	7.99	8.03
CC(1/2)	0.997	0.954

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1 M HEPES pH 7.5, 1.5 M Li2SO4