6IPA
C-terminal EMAP II-like domain of p43 refined against twinned data
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.170, 146.900, 146.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.730 - 2.470 |
| R-factor | 0.2056 |
| Rwork | 0.202 |
| R-free | 0.26110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zkg |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.783 |
| Data reduction software | DIALS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.750 | 2.510 |
| High resolution limit [Å] | 2.470 | 2.470 |
| Rmeas | 0.181 | 1.892 |
| Number of reflections | 29903 | 1455 |
| <I/σ(I)> | 12 | 1.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.8 | 11.3 |
| CC(1/2) | 1.000 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 12.5% w/v PEG 1000, 12.5% w/v PEG 3350, 12.5% v/v MPD, 0.02M amino acids, 0.1M MES |
