6IOU
The ligand binding domain of Mlp24 with serine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2016-06-24 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.670, 62.320, 73.170 |
| Unit cell angles | 90.00, 105.55, 90.00 |
Refinement procedure
| Resolution | 39.349 - 2.100 |
| R-factor | 0.2009 |
| Rwork | 0.198 |
| R-free | 0.25570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.167 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.400 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.091 | 0.395 |
| Number of reflections | 26828 | 2186 |
| <I/σ(I)> | 10.2 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1 M Tris pH 8.5, 33.5% (w/v) PEG 4000 |
