6IOT
The ligand binding domain of Mlp24 with arginine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2013-04-19 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.530, 102.750, 93.410 |
| Unit cell angles | 90.00, 94.51, 90.00 |
Refinement procedure
| Resolution | 48.483 - 2.700 |
| R-factor | 0.2193 |
| Rwork | 0.217 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.001 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.500 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.103 | 0.359 |
| Number of reflections | 32455 | 4264 |
| <I/σ(I)> | 8.7 | 3.7 |
| Completeness [%] | 97.1 | 96.2 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1M MES pH 6.0, 45% (v/v) PEG 400, 0.1M calcium acetate |
