6IOE
Crystal structure of the CysR-CTLD2 fragment of human MR at basic pH (pH 8.5)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NFPSS BEAMLINE BL18U |
Synchrotron site | NFPSS |
Beamline | BL18U |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2017-12-26 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 241.425, 72.240, 109.529 |
Unit cell angles | 90.00, 114.51, 90.00 |
Refinement procedure
Resolution | 29.846 - 2.900 |
R-factor | 0.239 |
Rwork | 0.236 |
R-free | 0.28420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xts |
RMSD bond length | 0.011 |
RMSD bond angle | 1.222 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.142 | 0.463 |
Number of reflections | 37435 | 3452 |
<I/σ(I)> | 11.71 | |
Completeness [%] | 97.5 | |
Redundancy | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 1.2 M potassium sodium tartrate tetrahydrate, 0.1 M Tris (pH 8.5) |