6IOD
The structure of UdgX in complex with single-stranded DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-23 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 43.805, 83.862, 122.664 |
| Unit cell angles | 90.00, 94.51, 90.00 |
Refinement procedure
| Resolution | 41.931 - 1.660 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.21690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6io9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.633 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.720 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.052 | 0.496 |
| Number of reflections | 52269 | 5176 |
| <I/σ(I)> | 33.1 | |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 6.8 | 6.8 |
| CC(1/2) | 0.998 | 0.944 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | 2.15M sodium malonate pH 7.0 and 3.8% MPD |
