6IOC
The structure of the H109Q mutant of UdgX in complex with uracil
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-23 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.683, 50.711, 38.826 |
| Unit cell angles | 90.00, 90.62, 90.00 |
Refinement procedure
| Resolution | 30.827 - 1.624 |
| R-factor | 0.1561 |
| Rwork | 0.155 |
| R-free | 0.18170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ioa |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.014 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.650 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.120 | 0.570 |
| Number of reflections | 18944 | 914 |
| <I/σ(I)> | 14.9 | 3.8 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 6 | 4.7 |
| CC(1/2) | 0.973 | 0.876 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 2.15M Sodium malonate pH 7.0 and 3.8% MPD |
