6IOB
The structure of the H109A mutant of UdgX in complex with uracil
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-23 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.151, 51.158, 40.957 |
| Unit cell angles | 90.00, 92.19, 90.00 |
Refinement procedure
| Resolution | 31.959 - 2.002 |
| R-factor | 0.1973 |
| Rwork | 0.195 |
| R-free | 0.25430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.874 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.102 | 0.473 |
| Number of reflections | 11019 | 1072 |
| <I/σ(I)> | 14 | 3 |
| Completeness [%] | 97.4 | 97.8 |
| Redundancy | 5 | 4 |
| CC(1/2) | 0.994 | 0.958 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 2.15M Sodium malonate pH 7.0 and 3.8% MPD |
