6IOA
The structure of UdgX in complex with uracil
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-03-01 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.407, 51.448, 55.430 |
| Unit cell angles | 90.00, 105.48, 90.00 |
Refinement procedure
| Resolution | 24.650 - 2.150 |
| R-factor | 0.16529 |
| Rwork | 0.163 |
| R-free | 0.21211 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6io9 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.116 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.650 | 2.210 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.066 | 0.150 |
| Number of reflections | 10655 | 1707 |
| <I/σ(I)> | 9.9 | 4.3 |
| Completeness [%] | 98.4 | 97.1 |
| Redundancy | 2.3 | 1.4 |
| CC(1/2) | 0.996 | 0.869 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | 0.05M MES pH 5.6, 2.45M (NH4)2SO4 and 0.1M MgCl2 |
