6IO6

Silver-bound Glyceraldehyde-3-phosphate dehydrogenase A at non-catalytic site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	I 41 2 2
Unit cell lengths	122.260, 122.260, 157.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.321 - 2.640
R-factor	0.2042
Rwork	0.202
R-free	0.24280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gad
Data reduction software	xia2
Data scaling software	Aimless (0.6.2)
Phasing software	PHASER (2.7.2)
Refinement software	PHENIX ((1.14_3260:000))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.320	48.320	2.710
High resolution limit [Å]	2.640	11.810	2.640
Rmerge	0.251	0.141	3.354
Rmeas	0.256	0.144	3.432
Rpim	0.051	0.031	0.689
Total number of observations	480506	5174	35628
Number of reflections	17924	243	1315
<I/σ(I)>	8.8	14.8	2
Completeness [%]	99.9	98.3	99.4
Redundancy	26.8	21.3	27.1
CC(1/2)	0.973	0.978	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.1M Malic acid, PEG3350 20%