6INF
a glycosyltransferase complex with UDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 97.662, 97.662, 89.626 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.830 - 1.690 |
| R-factor | 0.1797 |
| Rwork | 0.178 |
| R-free | 0.20744 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pq6 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.489 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.580 | 1.730 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.069 | 1.007 |
| Rmeas | 0.072 | 1.061 |
| Rpim | 0.022 | 0.333 |
| Number of reflections | 56938 | 4173 |
| <I/σ(I)> | 20.2 | 4 |
| Completeness [%] | 100.0 | |
| Redundancy | 19.2 | |
| CC(1/2) | 0.999 | 0.948 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M sodium citrate buffer at pH 5.4 and 20% PEG 4000 |
