6IN4
Crystal structure of apo DAPK1 in the presence of 18-crown-6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-02-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.675, 62.360, 88.428 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.420 - 1.800 |
R-factor | 0.1872 |
Rwork | 0.186 |
R-free | 0.21800 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.835 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.12-2829_1069: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.210 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 24036 | |
<I/σ(I)> | 17.5 | |
Completeness [%] | 97.8 | |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.8 M ammonium sulfate, 0.1 M MES pH 6.5, 100 mM 18-crown-6 |