6IMP
Crystal structure of alpha-beta hydrolase (ABH) from Vibrio vulnificus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-19 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.106, 99.681, 164.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.840 - 2.620 |
R-factor | 0.23986 |
Rwork | 0.237 |
R-free | 0.28701 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.430 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.077 | 0.597 |
Number of reflections | 22210 | |
<I/σ(I)> | 23.1 | 1.87 |
Completeness [%] | 98.2 | 97.4 |
Redundancy | 6.1 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M sodium formate, 25% PEG 3350 |