6IMF
Crystal structure of TOXIN/ANTITOXIN complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.90 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.166, 48.113, 75.028 |
| Unit cell angles | 90.00, 102.61, 90.00 |
Refinement procedure
| Resolution | 39.640 - 2.300 |
| R-factor | 0.187 |
| Rwork | 0.186 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1WVR AND 3IX0 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.515 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.508 | |
| Number of reflections | 17522 | |
| <I/σ(I)> | 11.488 | 2.434 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | AMMONIUM SULPHATE, MES, PEG8000, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K |
