6IM4

Structural basis of AimP signaling molecule recognition by AimR in Spbeta group of bacteriophages

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-12-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979183
Spacegroup name	P 21 21 2
Unit cell lengths	121.635, 214.153, 33.852
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.565 - 1.930
R-factor	0.2207
Rwork	0.219
R-free	0.26830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.869
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.565	2.030
High resolution limit [Å]	1.928	1.930
Rmerge	0.106
Number of reflections	135748
<I/σ(I)>	2
Completeness [%]	96.7
Redundancy	10
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291.5	PEG 4000 magnesium acetate