6IM0
Crystal structure of a highly thermostable carbonic anhydrase from Persephonella marina EX-H1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2018-05-15 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 124.885, 109.034, 123.186 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 123.190 - 2.600 |
| R-factor | 0.19183 |
| Rwork | 0.190 |
| R-free | 0.22719 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6eki |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.814 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 123.190 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rpim | 0.083 | 0.318 |
| Number of reflections | 47255 | 4968 |
| <I/σ(I)> | 9.8 | |
| Completeness [%] | 97.9 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.955 | 0.758 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 100 mM sodium HEPES pH 7.5, 28-32% (w/v) polyethylene glycol (PEG) 400, and 200-400 mM calcium chloride dehydrate. |
