6IKO

Crystal structure of mouse GAS7cb

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-19
Detector	RAYONIX MX300HE
Wavelength(s)	0.90000
Spacegroup name	P 21 3
Unit cell lengths	194.182, 194.182, 194.182
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.769 - 3.756
R-factor	0.1761
Rwork	0.173
R-free	0.20470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ikn
RMSD bond length	0.012
RMSD bond angle	1.566
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.810
High resolution limit [Å]	3.750	3.750
Number of reflections	25184	1253
<I/σ(I)>	22.4
Completeness [%]	99.9
Redundancy	20.8
CC(1/2)	0.990	0.560

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5	293	0.1 M sodium citrate (pH 5.0), 1.2 M sodium formate, 0.2 M NaCl, 1-4% ethylene glycol